c albicans atcc 44829 Search Results


c albicans atcc 44829  (ATCC)
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ATCC c albicans atcc 44829
C Albicans Atcc 44829, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c albicans  (ATCC)
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ATCC c albicans
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1 569 c albicans atcc 44829 c tropicalis atcc 750 c krusei atcc 6258 control  (ATCC)
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ATCC 1 569 c albicans atcc 44829 c tropicalis atcc 750 c krusei atcc 6258 control
1 569 C Albicans Atcc 44829 C Tropicalis Atcc 750 C Krusei Atcc 6258 Control, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc type strains  (ATCC)
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rab11 dsred  (Addgene inc)
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FIGURE 4. Internalized CTLA-4 recycles to the plasma membrane. A, CHO cells expressing CTLA-4 were transfected with <t>Rab11-mCherry</t> (red). Cells were then incubated with Alexa488-conjugated anti-CTLA-4 (green) for 30 min at 37 °C and observed by confocal microscopy. The white arrowheads indicate co-localization. B, CHO cells expressing CTLA-4 were labeled for 1 h with unconjugated mouse anti-CTLA-4. Cells were then placed on ice and surface CTLA-4-labeled with Alexa488 anti-mouse IgG (green). To observe recycling receptors, cells were then washed and labeled with Alexa555 anti-mouse IgG (red) at 37 °C for 45 min and fixed before confocal analysis. Control conditions are shown (left) where the Alexa555 antibody was incubated at 4 °C compared with incubation at 37 °C (right). C, flow cytometric analysis of recycling CTLA-4 is shown. Cells were labeled with mouse anti-CTLA-4 PE at 37 °C to detect cycling CTLA-4, which was followed by labeling of re-cycling protein with Alexa647 anti-mouse IgG at 4 or 37 °C for the indicated time-points. D, live-cell confocal imaging of recycling receptors was carried out using CTLA-4-expressing CHO cells. Cells were incubated with Alexa488-conjugated anti-CTLA-4 for 1 h at 37 °C. Cells were then washed, and surface CTLA-4 receptors were blocked with unconjugated anti-human IgG. Cells were then incubated with Alexa555-conjugated anti-human IgG to detect recycling receptors. Confocal Z-stacks were then acquired at the time points shown. Panel E shows quantification of cells from D. Cells were outlined in Image J, and the recycling mean pixel fluorescence is plotted against time. Error bars show S.E.
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anti chd5  (Cell Signaling Technology Inc)
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FIGURE 4. Internalized CTLA-4 recycles to the plasma membrane. A, CHO cells expressing CTLA-4 were transfected with <t>Rab11-mCherry</t> (red). Cells were then incubated with Alexa488-conjugated anti-CTLA-4 (green) for 30 min at 37 °C and observed by confocal microscopy. The white arrowheads indicate co-localization. B, CHO cells expressing CTLA-4 were labeled for 1 h with unconjugated mouse anti-CTLA-4. Cells were then placed on ice and surface CTLA-4-labeled with Alexa488 anti-mouse IgG (green). To observe recycling receptors, cells were then washed and labeled with Alexa555 anti-mouse IgG (red) at 37 °C for 45 min and fixed before confocal analysis. Control conditions are shown (left) where the Alexa555 antibody was incubated at 4 °C compared with incubation at 37 °C (right). C, flow cytometric analysis of recycling CTLA-4 is shown. Cells were labeled with mouse anti-CTLA-4 PE at 37 °C to detect cycling CTLA-4, which was followed by labeling of re-cycling protein with Alexa647 anti-mouse IgG at 4 or 37 °C for the indicated time-points. D, live-cell confocal imaging of recycling receptors was carried out using CTLA-4-expressing CHO cells. Cells were incubated with Alexa488-conjugated anti-CTLA-4 for 1 h at 37 °C. Cells were then washed, and surface CTLA-4 receptors were blocked with unconjugated anti-human IgG. Cells were then incubated with Alexa555-conjugated anti-human IgG to detect recycling receptors. Confocal Z-stacks were then acquired at the time points shown. Panel E shows quantification of cells from D. Cells were outlined in Image J, and the recycling mean pixel fluorescence is plotted against time. Error bars show S.E.
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type strains  (ATCC)
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ATCC type strains
FIGURE 4. Internalized CTLA-4 recycles to the plasma membrane. A, CHO cells expressing CTLA-4 were transfected with <t>Rab11-mCherry</t> (red). Cells were then incubated with Alexa488-conjugated anti-CTLA-4 (green) for 30 min at 37 °C and observed by confocal microscopy. The white arrowheads indicate co-localization. B, CHO cells expressing CTLA-4 were labeled for 1 h with unconjugated mouse anti-CTLA-4. Cells were then placed on ice and surface CTLA-4-labeled with Alexa488 anti-mouse IgG (green). To observe recycling receptors, cells were then washed and labeled with Alexa555 anti-mouse IgG (red) at 37 °C for 45 min and fixed before confocal analysis. Control conditions are shown (left) where the Alexa555 antibody was incubated at 4 °C compared with incubation at 37 °C (right). C, flow cytometric analysis of recycling CTLA-4 is shown. Cells were labeled with mouse anti-CTLA-4 PE at 37 °C to detect cycling CTLA-4, which was followed by labeling of re-cycling protein with Alexa647 anti-mouse IgG at 4 or 37 °C for the indicated time-points. D, live-cell confocal imaging of recycling receptors was carried out using CTLA-4-expressing CHO cells. Cells were incubated with Alexa488-conjugated anti-CTLA-4 for 1 h at 37 °C. Cells were then washed, and surface CTLA-4 receptors were blocked with unconjugated anti-human IgG. Cells were then incubated with Alexa555-conjugated anti-human IgG to detect recycling receptors. Confocal Z-stacks were then acquired at the time points shown. Panel E shows quantification of cells from D. Cells were outlined in Image J, and the recycling mean pixel fluorescence is plotted against time. Error bars show S.E.
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FIGURE 4. Internalized CTLA-4 recycles to the plasma membrane. A, CHO cells expressing CTLA-4 were transfected with Rab11-mCherry (red). Cells were then incubated with Alexa488-conjugated anti-CTLA-4 (green) for 30 min at 37 °C and observed by confocal microscopy. The white arrowheads indicate co-localization. B, CHO cells expressing CTLA-4 were labeled for 1 h with unconjugated mouse anti-CTLA-4. Cells were then placed on ice and surface CTLA-4-labeled with Alexa488 anti-mouse IgG (green). To observe recycling receptors, cells were then washed and labeled with Alexa555 anti-mouse IgG (red) at 37 °C for 45 min and fixed before confocal analysis. Control conditions are shown (left) where the Alexa555 antibody was incubated at 4 °C compared with incubation at 37 °C (right). C, flow cytometric analysis of recycling CTLA-4 is shown. Cells were labeled with mouse anti-CTLA-4 PE at 37 °C to detect cycling CTLA-4, which was followed by labeling of re-cycling protein with Alexa647 anti-mouse IgG at 4 or 37 °C for the indicated time-points. D, live-cell confocal imaging of recycling receptors was carried out using CTLA-4-expressing CHO cells. Cells were incubated with Alexa488-conjugated anti-CTLA-4 for 1 h at 37 °C. Cells were then washed, and surface CTLA-4 receptors were blocked with unconjugated anti-human IgG. Cells were then incubated with Alexa555-conjugated anti-human IgG to detect recycling receptors. Confocal Z-stacks were then acquired at the time points shown. Panel E shows quantification of cells from D. Cells were outlined in Image J, and the recycling mean pixel fluorescence is plotted against time. Error bars show S.E.

Journal: Journal of Biological Chemistry

Article Title: Constitutive Clathrin-mediated Endocytosis of CTLA-4 Persists during T Cell Activation

doi: 10.1074/jbc.m111.304329

Figure Lengend Snippet: FIGURE 4. Internalized CTLA-4 recycles to the plasma membrane. A, CHO cells expressing CTLA-4 were transfected with Rab11-mCherry (red). Cells were then incubated with Alexa488-conjugated anti-CTLA-4 (green) for 30 min at 37 °C and observed by confocal microscopy. The white arrowheads indicate co-localization. B, CHO cells expressing CTLA-4 were labeled for 1 h with unconjugated mouse anti-CTLA-4. Cells were then placed on ice and surface CTLA-4-labeled with Alexa488 anti-mouse IgG (green). To observe recycling receptors, cells were then washed and labeled with Alexa555 anti-mouse IgG (red) at 37 °C for 45 min and fixed before confocal analysis. Control conditions are shown (left) where the Alexa555 antibody was incubated at 4 °C compared with incubation at 37 °C (right). C, flow cytometric analysis of recycling CTLA-4 is shown. Cells were labeled with mouse anti-CTLA-4 PE at 37 °C to detect cycling CTLA-4, which was followed by labeling of re-cycling protein with Alexa647 anti-mouse IgG at 4 or 37 °C for the indicated time-points. D, live-cell confocal imaging of recycling receptors was carried out using CTLA-4-expressing CHO cells. Cells were incubated with Alexa488-conjugated anti-CTLA-4 for 1 h at 37 °C. Cells were then washed, and surface CTLA-4 receptors were blocked with unconjugated anti-human IgG. Cells were then incubated with Alexa555-conjugated anti-human IgG to detect recycling receptors. Confocal Z-stacks were then acquired at the time points shown. Panel E shows quantification of cells from D. Cells were outlined in Image J, and the recycling mean pixel fluorescence is plotted against time. Error bars show S.E.

Article Snippet: Co-localization—For Rab11 co-localization, CHO cells expressing CTLA-4 were co-transfected with Rab11 DsRed (Addgene) (26) and plated on glass-bottom dishes overnight.

Techniques: Clinical Proteomics, Membrane, Expressing, Transfection, Incubation, Confocal Microscopy, Labeling, Control, Imaging, Fluorescence